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| Registros recuperados : 1 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
17/07/2023 |
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Data da última atualização: |
27/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
GOMES, T. C.; CONRADO, R.; OLIVEIRA, R. C. de; SELARI, P. J. R. G.; MELO, I. S. de; ARAÚJO, W. L.; MARIA, D. A.; SOUZA, A. O. de. |
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Afiliação: |
TAINAH COLOMBO GOMES, INSTITUTO BUTANTAN; RAFAEL CONRADO, INSTITUTO BUTANTAN; RODRIGO CARDOSO DE OLIVEIRA, UNIVERSIDADE DE SÃO PAULO; PRISCILA JANE ROMANO GONÇALVES SELARI, INSTITUTO FEDERAL GOIANO; ITAMAR SOARES DE MELO, CNPMA; WELINGTON LUIZ ARAÚJO, UNIVERSIDADE DE SÃO PAULO; DURVANEI AUGUSTO MARIA, INSTITUTO BUTANTAN; ANA OLÍVIA DE SOUZA, INSTITUTO BUTANTAN. |
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Título: |
Effect of Monocerin, a fungal secondary metabolite, on endothelial cells. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Toxins v. 15, n. 5, article 344, 2023. |
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ISSN: |
2072-6651 |
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DOI: |
https://doi.org/10.3390/toxins15050344 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study reports the isolation and identification of the endophytic fungus Exserohilum rostratum through molecular and morphological analysis using optical and transmission electron microscopy (TEM), as well as the procurement of its secondary metabolite monocerin, an isocoumarin derivative. Considering the previously observed biological activities of monocerin, this study was performed on human umbilical vein endothelial cells (HUVECs) that are widely used as an in vitro model for several different purposes. Important parameters, such as cell viability, senescence-associated ?-galactosidase, cellular proliferation by using 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE), apoptosis analysis with annexin, cellular morphology through scanning electron microscopy (SEM), and laser confocal analysis were evaluated after exposing the cells to monocerin. After 24 h of exposure to monocerin at 1.25 mM, there was more than 80% of cell viability and a low percentage of cells in the early and late apoptosis and necrosis. Monocerin increased cell proliferation and did not induce cell senescence. Morphological analysis showed cellular integrity. The study demonstrates aspects of the mechanism of action of monocerin on endothelial cell proliferation, suggesting the possibility of its pharmaceutical application, such as in regenerative medicine. |
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Palavras-Chave: |
Monocerin. |
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Thesagro: |
Caatinga; Cróton; Fungo. |
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Thesaurus NAL: |
Cell senescence; Cell viability; Endophytes; Exserohilum; Fungi; Medicinal fungi; Secondary metabolites. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154958/1/Melo-Effect-monocerin-2022.pdf
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Marc: |
LEADER 02351naa a2200361 a 4500
001 2154958
005 2023-07-27
008 2023 bl uuuu u00u1 u #d
022 $a2072-6651
024 7 $ahttps://doi.org/10.3390/toxins15050344$2DOI
100 1 $aGOMES, T. C.
245 $aEffect of Monocerin, a fungal secondary metabolite, on endothelial cells.$h[electronic resource]
260 $c2023
520 $aAbstract: This study reports the isolation and identification of the endophytic fungus Exserohilum rostratum through molecular and morphological analysis using optical and transmission electron microscopy (TEM), as well as the procurement of its secondary metabolite monocerin, an isocoumarin derivative. Considering the previously observed biological activities of monocerin, this study was performed on human umbilical vein endothelial cells (HUVECs) that are widely used as an in vitro model for several different purposes. Important parameters, such as cell viability, senescence-associated ?-galactosidase, cellular proliferation by using 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE), apoptosis analysis with annexin, cellular morphology through scanning electron microscopy (SEM), and laser confocal analysis were evaluated after exposing the cells to monocerin. After 24 h of exposure to monocerin at 1.25 mM, there was more than 80% of cell viability and a low percentage of cells in the early and late apoptosis and necrosis. Monocerin increased cell proliferation and did not induce cell senescence. Morphological analysis showed cellular integrity. The study demonstrates aspects of the mechanism of action of monocerin on endothelial cell proliferation, suggesting the possibility of its pharmaceutical application, such as in regenerative medicine.
650 $aCell senescence
650 $aCell viability
650 $aEndophytes
650 $aExserohilum
650 $aFungi
650 $aMedicinal fungi
650 $aSecondary metabolites
650 $aCaatinga
650 $aCróton
650 $aFungo
653 $aMonocerin
700 1 $aCONRADO, R.
700 1 $aOLIVEIRA, R. C. de
700 1 $aSELARI, P. J. R. G.
700 1 $aMELO, I. S. de
700 1 $aARAÚJO, W. L.
700 1 $aMARIA, D. A.
700 1 $aSOUZA, A. O. de
773 $tToxins$gv. 15, n. 5, article 344, 2023.
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Embrapa Meio Ambiente (CNPMA) |
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